Kaustuv Datta

Research Summary

Proper mitochondrial biogenesis and its optimal functioning are critical to numerous cellular processes including adaptation to nutrient availability and stress. Mitochondria require coordinated expression of nuclear and its own genome, thus requiring its own translation apparatus in addition to its cytosolic counterpart. Metabolic activity of mitochondria vis-à-vis ATP generation is altered between rapidly proliferating cells and differentiated cells. ATP generation via glycolysis is the preferred pathway in proliferating cells such as tumor/stem cells where mitochondrial activity is maintained at a minimal level. Respiration via mitochondria is the preferred pathway in differentiated cells. Conversion of proliferating cells to differentiated cells is contingent on smooth transition of the mitochondrial metabolic state and its dysregulation is thought to be a leading cause of diseases including cancer. Saccharomyces cerevisiae life cycle mimics these metabolic states and the transition event. Cells generateATP through glycolysis in presence of glucose and oxygen where mitochondrial activity vis-à-vis ATP generation is minimal. Metabolic state of yeast cells at this state is akin to rapidly proliferating cells. Upon glucose exhaustion cells switch to utilizing ethanol a byproduct of fermentation via respiration in the mitochondria to generate ATP. Metabolic state of the yeast cells at this point is akin to differentiated cells. My lab is interested understanding the molecules that regulate the mitochondrial gene expression especially during the transition from glycolysis only to respiration in yeast. Factors involved in this simple switch are likely conserved in yeast and humans.

 
Major Research Findings
 
  • Our lab has identified a GTPase localized to the mitochondrial ribosomes that is likely to respond to changes in ATP/ADP ratio and regulate cells ability to carry out respiration.
  • Our lab has identified a separate GTPase that aids in joining of large and small mitochondrial ribosomal subunits.
 
Recent Publications
 

Huber M.D, Vesely P.W., Datta K and Gerace L. (2013) Erlins restrict SREBP activation in the ER and regulate cellular cholesterol homeostasis. J. Cell Biol. 203(3): 427–436

Datta K, Guan T, Gerace L.NET37, a nuclear envelope transmembrane protein with glycosidase homology, is involved in myoblast differentiationJ Biol Chem. 2009 Oct 23;284(43):29666-76

Liu G, Guan T, Datta K, Coppinger J, Yates J3rd, Gerace L.Regulation of Myoblast Differentiation by the Nuclear Envelope Protein NET39. Mol Cell Biol. 2009 Nov;29(21):5800-12.

Fuentes JL, Datta K, Sullivan SM, Walker A, Maddock JR. (2007) In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics. 278(1): 105-23.

Datta K, Fuentes JL, Maddock JR. (2005)The yeast GTPase Mtg2p is required for mitochondrial translation and partially suppresses an rRNA methyltransferase mutant, mrm2. Mol. Biol. Cell. 16(2):954-63.

Sikora AE, Datta K, Maddock JR. (2006) Biochemical properties of the Vibrio harveyi CgtAV GTPase. Biochem. Biophys. Res. Commun. 339(4):1165-70.

Sikora AE, Zielke R, Datta K, Maddock JR. (2006) The Vibrio harveyi GTPase CgtAV is essential and is associated with the 50S ribosomal subunit. J. Bacteriol. 188(3):1205-10.

Jiang M, Datta K, Walker A, Strahler J, Bagamasbad P, Andrews PC, Maddock JR. (2006) The Escherichia coli GTPase CgtAE is involved in late steps of large ribosome assembly. J. Bacteriol. 188(19): 6757-70.

Datta K, Skidmore JM, Pu K, Maddock JR. (2004) The Caulobacter crescentus GTPase CgtAC is required for progression through the cell cycle and for maintaining 50S ribosomal subunit levels.Mol. Microbiol. 54(5):1379-92.

Current contact landline, mobile, email and fax

Cell: 91-9818658174

Landline: 91-1124119810

Email: kdatta@south.du.ac.in


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