Deepak Pental

The major activity of the group is the genetic improvement of oilseed mustard (Brassica juncea) through conventional and biotechnological approaches. The major achievement in the form of tangible product is the development of the first ever hybrid in mustard, DMH-1, which gives 20-30% higher yield than pure line varieties. Another major area of activity is the molecular mapping and marker-assisted breeding of agronomically important traits in mustard. Some of the significant achievements are development of high-density molecular map of mustard, molecular tagging of many important quality traits such as, erucic acid by SNPs in the candidate genes, seed coat color by microsatellite markers and seed glucosinolates by both candidate genes and anonymous markers and QTL dissection of quantitative traits pertaining to yield. A comparative map of B. juncea consisting of Arabidopsis thaliana single copy gene sequences was developed which is being used for tagging agronomically important genes and candidate gene identification. The group is actively involved in marker-assisted introgression of quality traits in B. juncea through conventional plant breeding and through large scale application of doubled haploid (DH). Recently, a Centre of Excellence on genome mapping and molecular breeding in Brassica has been granted by the Department of Biotechnology, Government of India.
Major Research Findings
Prof. Deepak Pental and Prof. A. K. Pradhan’s lab has developed several genomic resources – 8 different bi-parental mapping populations (RILs/DH) for genome and gene mapping in mustard. They have also constructed high-density molecular map (consisting of > 3500 markers) in mustard, and developed the SNP-maps in B. rapa and B. juncea through the use of NGS-based RNA data. Further, the SNP markers have been used for saturation mapping of several genomic regions harbouring genes related to yield and quality in mustard and for the identification of candidate gene(s) for some important traits in mustard.
Selected Publications
  1. Rout, K., Sharma, M., Gupta, V., Mukhopadhyay, A., Sodhi, Y. S.,  PentalD., and Pradhan, A. K. (2015) Deciphering allelic variations for seed glucosinolate traits in oilseed mustard (Brassica juncea) using two bi-parental mapping populations. Theor. Appl. Genet. 128: 657 - 666
  2. Yadava, S. K., Paritosh, K., Panjabi-Massand, P., Gupta, V., Chandra, A.,  Sodhi, Y. S., Pradhan, A. K., Pental, D.  Tetralocular ovary and high siliqua width in yellow sarson lines of Brassica rapa(subspecies trilocularis) are due to a mutation in Bra034340 gene, a homologue ofCLAVATA3 in Arabidopsis. Theor. Appl. Genet. DOI 10.1007/s00122-014-2382-z
  3. Paritosh, K., Gupta, V., Yadava, S. K., Singh, P., Pradhan, A. K. Pental, D. 2014.  RNA-seq based SNPs for mapping in Brassica juncea (AABB): Synteny analysis between the two constituent genomes A (from B. rapa) and B (from B. nigra) shows highly divergent gene block arrangement and unique block fragmentation patterns. BMC Genomics 15: 396
  4. Sarita Sharma, K Lakshmi Padmaja, Vibha Gupta, Kumar Paritosh, Akshay K Pradhan, Deepak Pental(2014) Two Plastid DNA Lineages—Rapa/Oleracea and Nigra—within the Tribe Brassiceae Can Be Best Explained by Reciprocal Crosses at Hexaploidy: Evidence from Divergence Times of the Plastid Genomes and R-Block Genes of the A and B Genomes of Brassica juncea. PloS One, 9 (4): e93260.
  5. Paritosh K., Yadava, S.K., Gupta, V., Panjabi-Massand, P., Sodhi, Y.S., Pradhan, A. K. Pental, D. 2013. RNA-seq based SNPs in some agronomically important oleiferous lines of Brassica rapa and their use for genome-wide linkage mapping and specific-region fine mapping. BMC Genomics 14: 463.
  6. Padmaja L. K., Agarwal, P., Gupta, V., Mukhopadhyay, A., Sodhi Y.S., Pental, D., Pradhan, A. K. 2013. Natural mutations in two homoeologous TT8 genes control yellow seed coat trait in allotetroploid Brassica juncea (AABB). Theor. Appl. Genet. PP. 1-9. Doi: 10.1007/s00122-013-222-6.
  7. Paritosh K., Pental, D., and Burma, P. K., 2013. Structural and transcriptional characterization of rbcS genes of cotton (Gossypium hirsutum). Plant Mol Biol Rep 31: 1176-1183.
  8. Yadava, S. K., Arumugam, N., Mukhopadhyay, A., Sodhi, Y. S., Gupta, V., Pental, D., Pradhan, A. K. 2012. QTL mapping of yield associated traits in Brassica juncea: Meta-analysis and epistatic interactions using two different crosses between east European and Indian gene pool lines. Theor. Appl. Genet. (125: 1553-1564).
  9. Jagannath, A., Sodhi, Y. S., Gupta, V., Mukhopadhyay, A., Arumugam, N., Singh, I., Rohtagi, S., Burma, P., Pradhan, A. K. and Pental, D. 2011. Eliminating expression of erucic acid-encoding loci allows the identification of ‘hidden’ QTL contributing to oil quality fractions and oil content in Brassica juncea (Indian mustard). Theor. Appl. Genet. 122: 1091-1103.
  10. Panjabi, P-M., Yadav, S. K., Sharma, P., Kaur, A., Kumar, A., Arumugam, N., Sodhi, Y. S., Mukhopadhyay, A., Gupta, V., Pradhan, A. K. and Pental, D. 2010. Molecular mapping reveals two independent loci conferring resistance to Albugo candida in the east European germplasm of oilseed mustard Brassica juncea. Theor. Appl. Genet. 121: 137-145.

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